|
KMID : 1140120100150010069
|
|
Cancer Prevention Research
2010 Volume.15 No. 1 p.69 ~ p.75
|
|
|
Protective Effects of Eggplant (Solanum melongena L.) against Oxidative Stress under In Vitro and Cellular System
|
|
Kim Boh-Kyung
Cho Yun-Ju
Cho Eun-Ju
|
|
|
Abstract
|
|
|
The protective effects of eggplant (Solanum melongena L.) against oxidative stress were investigated under in vitro and cellular system using LLC-PK1 renal epithelial cells. The eggplant were extracted with methanol (MeOH) and then fractionated into three different layers: chloroform (CH2Cl2), butanol (BuOH) and aqueous layers(H2O). The extract and fractions of eggplant showed strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. In particular, among the fractions, BuOH fraction exerted the strongest effect with the IC50 value of 4.53μg/ml. In addition, the fractions except for CH2Cl2 also showed nitric oxide (NO) scavenging effect. The BuOH fractions showed superoxide anion (O2
?) radical scavenging activity. Even though it was not concentration- dependent manner, BuOH fractions at the concentration of 1 mg/ml showed O2 ? scavenging effect of 44.01%. It also exerted strong hydroxyl radical scavenging activity. Moreover, under LLC-PK1 cellular model, the BuOH fraction ameliorated oxidative stress induced by 2,2’-azobis (2-amidinopropane) dihydrochloride, the generator of peroxyl radical. In addition, the protective effect of BuOH fraction from NO under cellular oxidative stress was also studied. The LLC-PK1 cells showed the decreases in cell viability by the treatment with generators of peroxynitrite, 3-morpholinosydnonimine, and its precursors NO and superoxide anion produced by sodium nitroprusside and pyrogallol, respectively. However, the BuOH fraction led to the significant
increases in the cell viability with dose-dependence. The present study suggests that eggplant would have the protective activity from radical-induced oxidative stress and its BuOH fraction would play the crucial role.
|
|
|
KEYWORD
|
|
|
Eggplant, LLC-PK1 cell, Oxidative stres
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|